4 ms·

TIL; it seems like a common protocol: > MtBSTFA derivatives are 104 times more stable to hydrolysis than their corresponding TMS derivatives and produce easily

TIL; it seems like a common protocol: > MtBSTFA derivatives are 104 times more stable to hydrolysis than their corresponding TMS derivatives and produce easily interpreted mass spectra for GC/MS. https://www.registech.com/derivatizing-reagents/silylation-reagents/mtbstfa/ https://www.registech.com/derivatizing-reagents/silylation-r... EDIT: Derek Lowe: https://www.science.org/content/blog-post/alkanes-mars https://www.science.org/content/blog-post/alkanes-mars (thx to https://news.ycombinator.com/item?id=43548673 https://news.ycombinator.com/item?id=43548673 )

gsf_emergency_2 2y ago
Right, right, I'd put MS into my Blind Spot because there has got to be a much cheaper, more reliable, more scaleable/generalizable, less prone to over-engineering/misinterpretation option for detecting trace compounds, yes? (Our noses do it, at a speed faster than n per decade!!*) [Seems JPL are more courageous^W patient^W adaptive than MMAANG!] Follows (p3) the actually interesting phrase: what'd they mean by that? What provoked the peer reviewers? "It is INTERNALLY ACCEPTED by the SAM team that a signal can be assessed from a S/N of 2 and the quantification is tentatively made from any detected m/z" On reflection on the list I gave of DARPA-likes.. [Where might our intuitions diverge?] Couple clues for it not being simply nostalgia: 1. The right question is closer to : why do breakthroughs announced by/awarded to MAANG these past decades not pass the optimal curiosity-agency smell test? Too teleological? Cheap conspiratorial answer is that they focused on investigations too expensive for academics to disprove/replicate. Less cheap answer is SV unwittingly replicated the structural issues of pre-lost-decade Japan (shareholder-supremacy? Overoptimistic metrics?) 2. Which brings me to: cultural premiums being nolonger affordable by gradstudents/good faith middle managers across the board (Is it related to "supervision debt"? Or "moderation-technik debt") *Related to the information bottleneck worked upon by my undersung hero Bialek?

gsf_emergency_2 2y ago

Right, right, I'd put MS into my Blind Spot because there has got to be a much cheaper, more reliable, more scaleable/generalizable, less prone to over-engineering/misinterpretation option for detecting trace compounds, yes? (Our noses do it, at a speed faster than n per decade!!*) [Seems JPL are more courageous^W patient^W adaptive than MMAANG!] Follows (p3) the actually interesting phrase: what'd they mean by that? What provoked the peer reviewers? "It is INTERNALLY ACCEPTED by the SAM team that a signal can be assessed from a S/N of 2 and the quantification is tentatively made from any detected m/z" On reflection on the list I gave of DARPA-likes.. [Where might our intuitions diverge?] Couple clues for it not being simply nostalgia: 1. The right question is closer to : why do breakthroughs announced by/awarded to MAANG these past decades not pass the optimal curiosity-agency smell test? Too teleological? Cheap conspiratorial answer is that they focused on investigations too expensive for academics to disprove/replicate. Less cheap answer is SV unwittingly replicated the structural issues of pre-lost-decade Japan (shareholder-supremacy? Overoptimistic metrics?) 2. Which brings me to: cultural premiums being nolonger affordable by gradstudents/good faith middle managers across the board (Is it related to "supervision debt"? Or "moderation-technik debt") *Related to the information bottleneck worked upon by my undersung hero Bialek?