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Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where
by shpongled 2y ago
Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where you use antibodies against those specific proteins to detect them. The Pon S row is Ponceau S, a dye that non-specifically detects all proteins - so it's used as a loading control, to make sure that the same amount of total protein is loaded in each lane of the gel.
- doctorpangloss 2y agoIs it conceivable that the control was run once because the key result came from the same run? I can see a reviewer asking for it in all three figures, whereas they may drafted it only in one
- gus_massa 2y agoThe horizontal label is fine, it says Pon S in all images. (I guess a wrong label would be obvious to detect for specialists.) The problem are the vertical labels In Figure 1e it says: "MT1+2", "MT2" and "MT1" In Figure 3a it says: "5'-CR1", "CR2" and "3'-UTR" In Figure 3b it says: "CR2", "CR3" and "CR4"
- deleted 2y ago[deleted]
- shpongled 2y agoBased on the images, it is inconceivable that these are from the same run (see the dramatically different levels of TRF-S in each gel. One column/lane = one sample). This isn't something that would be included because of a reviewer - loading controls are required to meaningfully interpret the results (e.g. the data is useless without such a control).