you can with dosbox/pcem or something similar (dont think modern xen handles 16bit virtualization) -- but you can definitely run Windows 2000 in a VM on Qubes,

you can with dosbox/pcem or something similar (dont think modern xen handles 16bit virtualization) -- but you can definitely run Windows 2000 in a VM on Qubes, but the windows tools are not supported on anything under 7