4 ms·
Can you buy the primers personally for a few selected regions of great variability, do the extraction and PCR, and pay for sending it off for sequencing persona
by azalemeth 2y ago
Can you buy the primers personally for a few selected regions of great variability, do the extraction and PCR, and pay for sending it off for sequencing personally? You don't really need whole genome coverage and I'm sure if you asked if you could use a few ml of lysis buffer nobody would care. The scariest part for me would be sticking a buccal swab in your wolfdog's mouth...
- _yb2s 2y agoYes, that could possibly work with some carefully selected Sanger sequencing- I'd have to start with finding region(s) that had something I expected to be able to meaningfully interpret, which itself wouldn't be trivial, especially if the dog ends up being some type of wolfdog hybrid or just generally a less domesticated 'archaic dog.' Thanks for the suggestion- I'll look into it.