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Does it matter which CRISPR or CRISPR-like method is applied; or is there in general a dynamic response to gene editing in DNA/RNA?
by westurner 2y ago
Does it matter which CRISPR or CRISPR-like method is applied; or is there in general a dynamic response to gene editing in DNA/RNA?
- ca_tech 2y agoThe article covers this and I think the title is a bit too general. It is a byproduct of how CRISPR works as it targets a specific sequence. In this case the sequence is also present in areas that were non-targeted. Essentially, the sequence was not unique so the process impacted other areas in unintended ways.
- westurner 2y ago> Here we evaluated diverse corrective CRISPR-based editing approaches that target the ΔGT mutation in NCF1, with the goal of evaluating post-editing genomic outcomes. These approaches include Cas9 ribonucleoprotein (RNP) delivery with single-stranded oligodeoxynucleotide (ssODN) repair templates [11,12], dead Cas9 (dCas9) shielding of NCF113, multiplex single-strand nicks using Cas9 nickase (nCas9) [14,15], or staggered double-strand breaks (DSBs) using Cas12a16. - "A NICER approach to genome editing with less mutations than CRISPR" (2023) [cas13d] https://news.ycombinator.com/item?id=37698196 https://news.ycombinator.com/item?id=37698196 : "Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning" (2023) https://www.nature.com/articles/s41587-023-01830-8 https://www.nature.com/articles/s41587-023-01830-8