28 ms·

Sounds great. But the focus on the competition with in-house sequencing is odd. When i was doing a PhD twenty years ago, we never even considered doing it in-ho

by twic 2y ago

Sounds great. But the focus on the competition with in-house sequencing is odd. When i was doing a PhD twenty years ago, we never even considered doing it in-house - i would just post tubes to a commercial sequencing service and get results back a few days later (or, in one case, take tubes to their office myself to save some time). So, Plasmidsaurus are actually winning against those services. It would be more useful to focus on why that is. It sounds like the two distinguishing factors are fast turnaround, and long reads. But there are other players offering basically the same service now, and faster or cheaper: https://eurofinsgenomics.com/en/products/nanopore-sequencing/whole-plasmid-sequencing/ https://eurofinsgenomics.com/en/products/nanopore-sequencing... https://plasmidsng.com/ https://plasmidsng.com/ So we'll see how that pans out.

divbzero 2y ago
My experience was similar when I was doing a lot of cloning 15 years ago: We would routinely send tubes to a commercial sequencing service. How Plasmidsaurus competes against those other services is indeed the more interesting question.
- kjkjadksj 2y ago
  Really hard for them to compete at certain institutions. Some of these sequencing reps show up every afternoon to collect the days samples. Prices are so low that margins are probably tracing paper thin. Then some places will even have their own sequencing core facility offering all of that service at cost with even better turnaround, sometimes that day.
- abcc8 2y ago
  When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature. I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.

by twic 2y ago

divbzero 2y ago

My experience was similar when I was doing a lot of cloning 15 years ago: We would routinely send tubes to a commercial sequencing service. How Plasmidsaurus competes against those other services is indeed the more interesting question.

kjkjadksj 2y ago
Really hard for them to compete at certain institutions. Some of these sequencing reps show up every afternoon to collect the days samples. Prices are so low that margins are probably tracing paper thin. Then some places will even have their own sequencing core facility offering all of that service at cost with even better turnaround, sometimes that day.
abcc8 2y ago
When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature. I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.