3 ms·
Memoir: A Novel System for Neural Lineage Tracing
- _Microft 2y agoFrom the paper: „[…] two powerful techniques are essential for MEMOIR: first, a set of genomic elements termed ‘barcoded scratchpad’, whose sequences can be specifically targeted and deleted by Cas9-gRNA, are integrated into the genome of cells. Therefore, for a single cell, its progeny inherit the alterations of the barcoded scratchpads, and all the daughter cells can be traced permanently.“