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Heh yeah, I split the area to be imaged into squares, and run autofocus in the middle of the square. Then use a 'snake' like XY pattern across that square to c
by anfractuosity 2y ago
Heh yeah, I split the area to be imaged into squares, and run autofocus in the middle of the square. Then use a 'snake' like XY pattern across that square to create images.
But I need to dramatically increase the speed of this process to use across a whole CD etc.
- svara 2y agoAs long as you're scanning over a perfectly flat, if tilted (relative to the microscope's focus plane) 2d plane, you don't need to autofocus much. Just measure the focus at a few points and fit the plane, then run the scan and apply the focus parameters from the fit.