3 ms·
Getting that Z Axis perfectly level across the run is going to be fun at this scale.
by ElCapitanMarkla 2y ago
Getting that Z Axis perfectly level across the run is going to be fun at this scale.
- anfractuosity 2y agoHeh yeah, I split the area to be imaged into squares, and run autofocus in the middle of the square. Then use a 'snake' like XY pattern across that square to create images. But I need to dramatically increase the speed of this process to use across a whole CD etc.
- svara 2y agoAs long as you're scanning over a perfectly flat, if tilted (relative to the microscope's focus plane) 2d plane, you don't need to autofocus much. Just measure the focus at a few points and fit the plane, then run the scan and apply the focus parameters from the fit.