With the RP2040 host port connected to your development host and the listed pins connected to the DUT, yes. However keep in mind that then those two computers

With the RP2040 host port connected to your development host and the listed pins connected to the DUT, yes. However keep in mind that then those two computers (development host and DUT) are galvanically coupled via the shared USB GND line. There's a risk of creating ground loops. If both are connected to utility power, then they better be on the same outlet and physically close.