3 ms·
> However, its amino-acid sequence is vastly different, matching less than 60% of the sequence of the most closely related fluorescent protein in its training d
by flobosg 2y ago
> However, its amino-acid sequence is vastly different, matching less than 60% of the sequence of the most closely related fluorescent protein in its training data set.
Not to downplay this achievement, but 60% sequence identity is nowhere near “vastly different”.
- trott 2y agoThe twilight zone is 20-35%: https://pubmed.ncbi.nlm.nih.gov/10195279/ https://pubmed.ncbi.nlm.nih.gov/10195279/ (Incidentally, the author was on my thesis committee, but this isn't precisely my field of expertise.)
- flobosg 2y agoProteins from the same family (and thus the same fold) can share less than 10% identity and still keep the same functionality as shown by e.g. profile Hidden Markov Model comparisons.