3 ms·
Add the EtBr after you microwave the agarose
by COGlory 2y ago
Add the EtBr after you microwave the agarose
- flobosg 2y agoAnd cover the flask with some shrink wrap (maybe with a tiny hole punched into it) to hinder steam release.
- sierra1011 2y agoI actually miss the stretchy shrink wrap, it was pretty useful in daily lab life
- flobosg 2y agoParafilm? 100% agreed. I’ve thought about buying a roll, which will probably last me for a lifetime. One of these days, maybe.
- sierra1011 2y agoYeah, that is always an option. However once boiling hot, if you introduce something into the flask it's more likely to create an explosion front. A bit like when someone supercools a bottle of water and then taps it to make it freeze; except with exploding sticky gel, and it's as hot as lava* *Not actually as hot as lava, but wow does it feel like it
- COGlory 2y agoI wouldn't worry about that. I've poured hundreds of gels and added the EtBr to it after microwaving it every single time. Never had anything close to that happen. Just use a sufficiently concentrated EtBr stock and you're adding like 45 µL to 45 mL.