3 ms·

...in rats: Subjects were young adult (3–5 months) and aged (22–24 months) male F344xBN F1 rats obtained from the National Institute on Aging rodent colony m

...in rats: Subjects were young adult (3–5 months) and aged (22–24 months) male F344xBN F1 rats obtained from the National Institute on Aging rodent colony managed by Charles River. F344xBN F1 rats are particularly useful for the study of aging and aging-associated conditions as aged rats of this strain remain relatively healthy and show good cognitive function at baseline. Unfortunately, female rats of this strain were not available from this or any other vendor at the time these studies were completed.

talldrinkofwhat 3y ago
Isn't the real mis-step of this study that they put the rats on a HFD 3 (three!) days before testing? I thought it was common knowledge that the transitory periods of ketosis elevate blood lipids until your body adapts to its new food source / arrives at a new equilibrium. I would think their conclusion should be "Taken together, the present data indicate that (!__consumption of__) -> <__transition onto__> a HFD prior to laparotomy prolongs the neuroinflammatory response to surgery and induces persistent memory deficits in young adult and aged rats. We demonstrated that both age groups who ate HFD for 3 days prior to surgery displayed robust cued-fear memory deficits, and aged rats additionally exhibited contextual memory deficits. These memory impairments were associated with exaggerated and prolonged (3 weeks post-surgery)" Which is just another way to say, don't inflame your entire body with a new diet prior to undergoing surgery (which in and of itself is massively stressful).
- hx8 3y ago
  > We have previously shown that surgery alone, or 3-days of HFD can each evoke sufficient neuroinflammation to cause memory deficits in aged, but not young rats. The surgery + transition onto HFD seem to be more damaging than either one alone, which makes sense that doing two harmful things is worse than doing one harmful thing.
leoh 3y ago
Dude. “In rats” is such an easy cliche. Should we create an LLM to post it on every HN story regarding animal studies? Rats are a decent model. Would you prefer we test hypotheses on your body to get started instead? Let’s look at the experimental methods and develop better intuitions about what can reasonably hypothetically extrapolated to humans and what doesn’t instead of just saying “in rats.”
ineedaj0b 3y ago
When you remove a rats arm, it cannot use it. This effect does not occur in humans because when you amputate a human’s arm they can still use it. Rats are unlike humans so feel free to amputate your arm. You will still be able use it because rats are not humans duh

talldrinkofwhat 3y ago

Isn't the real mis-step of this study that they put the rats on a HFD 3 (three!) days before testing? I thought it was common knowledge that the transitory periods of ketosis elevate blood lipids until your body adapts to its new food source / arrives at a new equilibrium. I would think their conclusion should be "Taken together, the present data indicate that (!__consumption of__) -> <__transition onto__> a HFD prior to laparotomy prolongs the neuroinflammatory response to surgery and induces persistent memory deficits in young adult and aged rats. We demonstrated that both age groups who ate HFD for 3 days prior to surgery displayed robust cued-fear memory deficits, and aged rats additionally exhibited contextual memory deficits. These memory impairments were associated with exaggerated and prolonged (3 weeks post-surgery)" Which is just another way to say, don't inflame your entire body with a new diet prior to undergoing surgery (which in and of itself is massively stressful).

hx8 3y ago
> We have previously shown that surgery alone, or 3-days of HFD can each evoke sufficient neuroinflammation to cause memory deficits in aged, but not young rats. The surgery + transition onto HFD seem to be more damaging than either one alone, which makes sense that doing two harmful things is worse than doing one harmful thing.

leoh 3y ago

Dude. “In rats” is such an easy cliche. Should we create an LLM to post it on every HN story regarding animal studies? Rats are a decent model. Would you prefer we test hypotheses on your body to get started instead? Let’s look at the experimental methods and develop better intuitions about what can reasonably hypothetically extrapolated to humans and what doesn’t instead of just saying “in rats.”

ineedaj0b 3y ago

When you remove a rats arm, it cannot use it. This effect does not occur in humans because when you amputate a human’s arm they can still use it. Rats are unlike humans so feel free to amputate your arm. You will still be able use it because rats are not humans duh