Growing mammalian cell spheroids in vitro was one of the highlights for me and my scientific career. So rewarding and interesting, so much more useful than mon

Growing mammalian cell spheroids in vitro was one of the highlights for me and my scientific career. So rewarding and interesting, so much more useful than monolayer cell cultures. The article is about neurons, but they are fascinating in cancer research also. You can grow fibroblasts together with cancer cells and they start making something like a real tumor and start exhibiting responses more like in vivo tumors. My favorite way to grow them was just seed the cells in special plates that the cells don't want to attach to and they just start attaching to one another and start making a colony of self-organizing cells floating in the media. A good overview here- https://cancerci.biomedcentral.com/articles/10.1186/s12935-021-01853-8 https://cancerci.biomedcentral.com/articles/10.1186/s12935-0...