Back in ~2014-2015 I was looking at making this (worked in Chang Liu's orthorep lab) - the problem was that I couldn't get my hands on the PRD1 phage (which is

Back in ~2014-2015 I was looking at making this (worked in Chang Liu's orthorep lab) - the problem was that I couldn't get my hands on the PRD1 phage (which is a fascinating phage - protein primed replication, lipid membrane coliphage). They were clever about how they bootstrapped the system - they just expressed a whole lot of terminal protein and hoped that some would attach. And it did in VERY rare events.