3 ms·
is crispr a big improvement here over AAVs or zinc fingies?
by sjkoelle 3y ago
is crispr a big improvement here over AAVs or zinc fingies?
- stanford_labrat 3y agoAAV or adeno-associated virus is a delivery method for getting cas9 mRNA (the code that says, make cas9 protein and do gene editing). Zinc finger nucleases are a similar class of dna editing proteins. In this specific experiment they chose to transfect cells with plasmid directly rather than transduce with virus.
- jryb 3y agoZFNs are difficult and slow to engineer. There are certainly tradeoffs but the fact that almost the entire industry is using CRISPR-based approaches tells you where things lie on balance