3 ms·

FDA shuts down enquiries about DNA contamination in Covid vaccines

5 points by hammock 3y ago

deadlinermusic 3y ago
Bullshit site. Bullshit article.
- superduty 3y ago
  [flagged]
bubaja 3y ago
What does this even mean? Can someone eli5?
- hammock 3y ago
  Someone altered the link to a paywalled version of the same article (for some reason). Here is the full article for free: https://brownstone.org/articles/fda-shuts-down-enquiries-about-dna-contamination-in-covid-vaccines/ https://brownstone.org/articles/fda-shuts-down-enquiries-abo...
- bubaja 3y ago
  [flagged]
  - hammock 3y ago
    Summarizing the key points made in the article (not my own opinions): Plasmid DNA is a byproduct inherent in a certain way of manufacturing vaccines. The FDA/WHO set a limit of 10 ng/dose of pDNA for vaccines due to the dangers that it can sometimes be incorporated into the patient's DNA and/or cause cancer. (For a bad example - https://en.wikipedia.org/wiki/Vaccine_contamination_with_SV40 https://en.wikipedia.org/wiki/Vaccine_contamination_with_SV4...) pDNA has recently been found in mRNA vaccines at levels far exceeding 10 ng/dose. How these doses got past the FDA scrutiny and into pharmacies is attributed to a change in manufacturing process to the DNA-byproduct process (away from the more expensive and time-intensive PCR process) happening after the safety data was submitted to the FDA. A review of the data of adverse events following vaccination signals that there may be an association with increased cancer rates. The FDA's response to the new information about pDNA being found is essentially that a)it's not the same pDNA that caused the polio vaccine scandal, and b)they don't have any concern and will not be applying more scrutiny than they already have to the whole ordeal
rolph 3y ago
until DNA sequences having features allowing cellular transfection [entry], and integration [inserting into host DNA] is found in vaccine, there is no basis for concern. specific modification of a sequence of DNA is required for transfection, and integration. this is not a random occurance, and even best case outcome, is less than 10% success, and requires supportive conditions.
- hammock 3y ago
  Perhaps you are right. It's an interesting area of research for sure, as the lipid nanoparticle (LNP) delivery system of these vaccines is new, and a lot of hypothesizing has been done that the residual DNA being encapsulated in the LNP makes it easier to transfect (the LNP is specifically designed to be able to enter cells) and resistant to the typical biological processes (nucleases) that would prevent integration
  - rolph 3y ago
    LNPs are actually better than that! they integrate with the cell membrane, and the mRNA moves through the cytoplasm. so each cell that is ~transfected with mRNA has signature lipids in the membrane
deleted 3y ago
[deleted]

5 points by hammock 3y ago

deadlinermusic 3y ago

Bullshit site. Bullshit article.

superduty 3y ago
[flagged]

bubaja 3y ago

What does this even mean? Can someone eli5?

hammock 3y ago
Someone altered the link to a paywalled version of the same article (for some reason). Here is the full article for free: https://brownstone.org/articles/fda-shuts-down-enquiries-about-dna-contamination-in-covid-vaccines/ https://brownstone.org/articles/fda-shuts-down-enquiries-abo...
bubaja 3y ago
[flagged]
- hammock 3y ago
  Summarizing the key points made in the article (not my own opinions): Plasmid DNA is a byproduct inherent in a certain way of manufacturing vaccines. The FDA/WHO set a limit of 10 ng/dose of pDNA for vaccines due to the dangers that it can sometimes be incorporated into the patient's DNA and/or cause cancer. (For a bad example - https://en.wikipedia.org/wiki/Vaccine_contamination_with_SV40 https://en.wikipedia.org/wiki/Vaccine_contamination_with_SV4...) pDNA has recently been found in mRNA vaccines at levels far exceeding 10 ng/dose. How these doses got past the FDA scrutiny and into pharmacies is attributed to a change in manufacturing process to the DNA-byproduct process (away from the more expensive and time-intensive PCR process) happening after the safety data was submitted to the FDA. A review of the data of adverse events following vaccination signals that there may be an association with increased cancer rates. The FDA's response to the new information about pDNA being found is essentially that a)it's not the same pDNA that caused the polio vaccine scandal, and b)they don't have any concern and will not be applying more scrutiny than they already have to the whole ordeal

rolph 3y ago

until DNA sequences having features allowing cellular transfection [entry], and integration [inserting into host DNA] is found in vaccine, there is no basis for concern. specific modification of a sequence of DNA is required for transfection, and integration. this is not a random occurance, and even best case outcome, is less than 10% success, and requires supportive conditions.

hammock 3y ago
Perhaps you are right. It's an interesting area of research for sure, as the lipid nanoparticle (LNP) delivery system of these vaccines is new, and a lot of hypothesizing has been done that the residual DNA being encapsulated in the LNP makes it easier to transfect (the LNP is specifically designed to be able to enter cells) and resistant to the typical biological processes (nucleases) that would prevent integration
- rolph 3y ago
  LNPs are actually better than that! they integrate with the cell membrane, and the mRNA moves through the cytoplasm. so each cell that is ~transfected with mRNA has signature lipids in the membrane

deleted 3y ago

[deleted]