4 ms·

thanks for sharing. since you sound like an expert, do you know if this technique works for live imaging of RNA molecules < 200 nucleotides? or would tagging

thanks for sharing. since you sound like an expert, do you know if this technique works for live imaging of RNA molecules < 200 nucleotides? or would tagging such a small molecule potentially alter biological processes and contaminate results? [edited to clarify live-imaging requirement]

w10-1 3y ago
FISH works for both DNA and RNA. When articles have pretty colors lighting up the inside of a cell, it's likely FISH. First google hit is a 2020 summary of RNA-FISH, "Technical review and guide to RNA fluorescence in situ hybridization": https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085896/
- panabee 3y ago
  thanks for the link. i saw this already; it's very useful. to clarify, the question was meant for live imaging and the risk of altering biological processes for target RNA molecules < 200 nucleotides.
foota 3y ago
I am not exactly an expert, I just happened to do a deep dive into microscopy techniques a couple years ago :-) The general term for these types of techniques (e.g., ones that let you image things below the "diffraction limit", which is roughly half the wavelength of light being used to image, see [1]), is super resolution microscopy[2]. There are a few other types you might find interesting. 1: https://en.wikipedia.org/wiki/Diffraction-limited_system https://en.wikipedia.org/wiki/Diffraction-limited_system 2: https://en.wikipedia.org/wiki/Super-resolution_microscopy https://en.wikipedia.org/wiki/Super-resolution_microscopy
- panabee 3y ago
  thanks for sharing, will check these out! based on your understanding, do you think it's possible to do live imaging of RNA molecules < 200 nucleotides -- without altering biological processes? super resolution microscopy references DNA imaging but doesn't delve into contamination risk, which is the critical bit. the first link didn't mention DNA/RNA applications at all. do you mind sharing the other types you recommend investigating?

w10-1 3y ago

FISH works for both DNA and RNA. When articles have pretty colors lighting up the inside of a cell, it's likely FISH. First google hit is a 2020 summary of RNA-FISH, "Technical review and guide to RNA fluorescence in situ hybridization": https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7085896/

panabee 3y ago
thanks for the link. i saw this already; it's very useful. to clarify, the question was meant for live imaging and the risk of altering biological processes for target RNA molecules < 200 nucleotides.

foota 3y ago

I am not exactly an expert, I just happened to do a deep dive into microscopy techniques a couple years ago :-) The general term for these types of techniques (e.g., ones that let you image things below the "diffraction limit", which is roughly half the wavelength of light being used to image, see [1]), is super resolution microscopy[2]. There are a few other types you might find interesting. 1: https://en.wikipedia.org/wiki/Diffraction-limited_system https://en.wikipedia.org/wiki/Diffraction-limited_system 2: https://en.wikipedia.org/wiki/Super-resolution_microscopy https://en.wikipedia.org/wiki/Super-resolution_microscopy

panabee 3y ago
thanks for sharing, will check these out! based on your understanding, do you think it's possible to do live imaging of RNA molecules < 200 nucleotides -- without altering biological processes? super resolution microscopy references DNA imaging but doesn't delve into contamination risk, which is the critical bit. the first link didn't mention DNA/RNA applications at all. do you mind sharing the other types you recommend investigating?