4 ms·
I must have misread some literature so thanks for clearing that up. I'm now seeing that it only requires a homologous template which, hypothetically could even
by heyoni 3y ago
I must have misread some literature so thanks for clearing that up. I'm now seeing that it only requires a homologous template which, hypothetically could even be provided by the therapeutic agent itself?
- dillydogg 3y agoYes, this is actually how prime editing works! You also attach some reverse transcriptase to perform the base insertion (but the repair requires cellular help, prime editing works in non-dividing cells though!)
- heyoni 3y agoIs prime editing possible for diseases that result from point mutations? I know tat there’s always the issue of getting the construct into the cells and then into the nucleus but can we confidently elicit base edits in humans? All I’ve seen (and I haven’t seen everything) is crispr mediated transcription in Duchene muscle dystrophy but it failed. I’m looking at possible cures for satb2 but was under the impression that NHEJ wasn’t viable for some reason (my notes suck).