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Filming is one really interesting application, the other is imaging of single proteins without the need for crystallisation. I wrote a whole PhD thesis about th
by benjaminva 3y ago
Filming is one really interesting application, the other is imaging of single proteins without the need for crystallisation. I wrote a whole PhD thesis about the idea how to reconstruct the three-dimensional electron density from a lot of extremely noisy single proteins shots. It will make a huge class of proteins experimentally accessible that we couldn't structurally look into before.
- pfdietz 3y agoHasn't cryoelectron microscopy pretty much taken over for protein structure determination?
- benjaminva 3y agoIndeed and it's a really impressive technology. However, there is the cooling involved and a lot of effort goes into sampling "the real deal" without changes of the structure due to the imaging process. In single molecule shots the idea is to form tiny droplets with the natural protein inside and sample them at room temperature. Naturally sample preparation here comes with its own challenges.
- ImPickleVic 3y agoDo you have links to related content?
- benjaminva 3y agoHere you go: https://www.nature.com/articles/s41467-018-04830-4 https://www.nature.com/articles/s41467-018-04830-4