> The sporozoite preparation came directly from the salivary glands of mosquitoes, which had been dissected, ground up, and irradiated. But how to purify this p

> The sporozoite preparation came directly from the salivary glands of mosquitoes, which had been dissected, ground up, and irradiated. But how to purify this preparation remained an open question. It mattered because waste material from mosquitoes’ salivary glands could be dangerous, causing embolisms or severe reactions if injected. I'm amazed that dissecting mosquitoes to extract malaria parasites for use in a vaccine actually produced enough material to test it on animals, and even more amazed that they've kept working on this approach since 1967. The article links to https://www.wired.com/story/tiny-guillotine-decapitates-mosquitoes-to-fight-malaria/ https://www.wired.com/story/tiny-guillotine-decapitates-mosq... which mentions that they had people dissecting 300 mosquitoes an hour before introducing some automation to handle 30 simultaneously. (It doesn't mention though how they deal with the dangerous waste material problem.)