> For derivation of TNT-hiPS cells, the day 7 reprogramming intermediates were transitioned to naive medium (t2iLGoY) instead. When dome-shaped colonies were ev

> For derivation of TNT-hiPS cells, the day 7 reprogramming intermediates were transitioned to naive medium (t2iLGoY) instead. When dome-shaped colonies were evident 5 days later, intermediate cells were collected using Accutase (Stem Cell Technologies) and reseeded onto a layer of iMEFs in naive conditions.