3 ms·
For anyone interested, the problem mostly boils down to the sequence lenght we can read. Modern sequencing techniques allow us o read 200 to 500 bases at a tim
by nzach 3y ago
For anyone interested, the problem mostly boils down to the sequence lenght we can read.
Modern sequencing techniques allow us o read 200 to 500 bases at a time. So after hat we need do find a way o arrange these short sequences into a single sequence. And this can be prety hard, especially when you are doing 'de novo' assembly[0].
Besides that, there is the fact that some regions of DNA are repeated[1].
[0] - https://en.wikipedia.org/wiki/Third-generation_sequencing#De_novo_assembly https://en.wikipedia.org/wiki/Third-generation_sequencing#De...
[1] - https://en.wikipedia.org/wiki/Repeated_sequence_(DNA) https://en.wikipedia.org/wiki/Repeated_sequence_(DNA)
- gww 3y agoYou are a bit behind on the available read lengths. The Oxford nanopore can produce reads as long as 300kb (k as in kilo) and the PacBio hifi can product 15 to 20kb reads. Finally a now defunct technology by 10x genomics called linked read sequencing also permitted longer regions to be sequences using short reads.
- nzach 3y agoThanks for pointing that out. My knowledge cut-off period in this domain is around 2018. So it's not suprising that things moved on.