3 ms·
Not true now with PacBio Revio and new Oxford Nanopore chemistry and flow cells. We still use a bit of older Illumina data for polishing, but long read methods
by robwwilliams 3y ago
Not true now with PacBio Revio and new Oxford Nanopore chemistry and flow cells. We still use a bit of older Illumina data for polishing, but long read methods now as accurate. HiC and Bionano more important as supplements than 150 nucleotide pair-end reads.
And the cost of a T2T using a combination of these technologies is already well under $10,000 in reagent costs. The assembly is still a complex art especially for a messy chromosome like Y.