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They relied mainly on PacBio HiFi reads (which I think is a truly understated revolutionary technology in genomics), then used Nanopore sequencing to link toget
by jakobnissen 3y ago
They relied mainly on PacBio HiFi reads (which I think is a truly understated revolutionary technology in genomics), then used Nanopore sequencing to link together the HiFi contigs to cover the truly gnarly regions of the chromosome.
And yeah, you're right: All modern large-scale sequencing is shotgun sequencing where the DNA is randomly broken up, each fragment is sequenced, and then the individual segments (reads) are assembled using a genome assembler.
- robwwilliams 3y agoAnd the PacBio system they used in this paper is already old news and has been replaced by PacBio Revio system. Higher throughput, cheaper per base and somewhat more accurate.