4 ms·
The original title is "Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression". Also, altho
by mutant_glofish 3y ago
The original title is "Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression".
Also, although I'm not sure that I understand this completely, it seems to me that this isn't quite a new development. For example, here's a 2016 paper claiming to do something very similar [1] (with the additional step of transplanting the human cells into mice): https://www.spandidos-publications.com/10.3892/mmr.2016.5191 https://www.spandidos-publications.com/10.3892/mmr.2016.5191
[1]: From the 2016 paper: "In the present study, human iPSCs were induced to differentiate into ovarian granulosa-like cells (OGLCs) in vitro. Subsequently, these cells were transplanted into POF mice."
- Metacelsus 3y agoHi, I'm the first author on the eLife paper. The 2016 paper you mentioned is basically worthless (their differentiation method doesn't make biological sense and didn't work when I tried it, they're only looking at a small subset of cherrypicked markers to conclude they have granulosa cells, and also, these people are from the "Shanghai University of Traditional Chinese Medicine"). We have much more functional validation of our granulosa-like cells, in terms of gene expression, hormone production, and follicle formation. A better precedent would be Yoshino 2021: https://pubmed.ncbi.nlm.nih.gov/34437124/ https://pubmed.ncbi.nlm.nih.gov/34437124/, which made mouse granulosa cells from mouse stem cells: