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I've always been curious about this. Can you briefly summarize how you take peripheral blood and fractionate it out to these small bits. What's the deal exactly
by jxramos 3y ago
I've always been curious about this. Can you briefly summarize how you take peripheral blood and fractionate it out to these small bits. What's the deal exactly? Is it like centrifuged and the plasma pulled out and everything is solution there or it's in the buffy coat or down deeper? Where are these proteins found exactly and how are they purified?
- jxramos 3y agosounds like this one would have been a great read https://pubmed.ncbi.nlm.nih.gov/3280421/ https://pubmed.ncbi.nlm.nih.gov/3280421/ Good overviews here https://comis.med.uvm.edu/vic/coursefiles/MD540/MD540-Protein_Methods_Learning_Module_10400_593281210/Protein-methods/Protein_Methods_print.html https://comis.med.uvm.edu/vic/coursefiles/MD540/MD540-Protei... and https://www.promega.com/resources/guides/protein-analysis/protein-purification-methods/ https://www.promega.com/resources/guides/protein-analysis/pr... I always thought proteins were too big for some of these techniques but apparently not.