3 ms·
I'm not sure about what's the problem with slow thawing though: AFAIK most tissues are pretty stable at fridge temperature for a few hours (from 4hours for a he
by littlestymaar 3y ago
I'm not sure about what's the problem with slow thawing though: AFAIK most tissues are pretty stable at fridge temperature for a few hours (from 4hours for a heart, up to 36hours for a kidney), but freezing is another matter: the slower you freeze things, the bigger the ice crystal grow, and IIRC you need to freeze things very quickly if you don't want damages from the ice crystal (you want to “vitrify” the water, instead of crystalizing it) and I don't think even liquide nitrogen is enough to freeze big organs quickly enough.
- AstralStorm 3y agoThere are some tricks with cryoprotectants and rarely used cryopreserving protein, but triggering that and not damaging cells is an active field of research.