4 ms·
Cool, but as described, this wouldn't work on humans. The light sheet microscopy technique that the suped-up MRI data is paired with to create these images requ
by pealco 3y ago
Cool, but as described, this wouldn't work on humans. The light sheet microscopy technique that the suped-up MRI data is paired with to create these images requires tissue to be "cleared" (or made transparent) with solvents, which obviously you can't do with a living human brain. To be honest, I don't quite understand how light sheet microscopy works with living _mouse_ brains.
- BenFranklin100 3y agoIt doesn’t. The brain must be cleared which generally involves removing lipids so the tissue has a homogeneous index of refraction throughout. Given lipids are an extremely important component of the brain (for instance myelin is a lipid rich material that surrounds axons) even if one were able to clear the brain without excising it from the skull, the clearing process essentially destroys the brain by preventing it from function properly.
- robwwilliams 3y agoCorrect. But that is where the lightsheet adds sufficient resolution. MRI for the “mesopic” scale, lightsheet for for microscopic scale to 0.2 microns, and selective elecron microscopy down as low as tissue quality will allow.
- BenFranklin100 3y agoYou’re not even responding to the original questions, which is is lightsheet microscopy capable of imaging entire living brains? Pay attention.
- kevviiinn 3y agoCan you use light microscopy on a living brain? No
- BenFranklin100 3y agoTwo and three photon microscopy works on living brains down to depth of approximately 1.5 mm. A company even sells head mounted miniature microscopes for mice. Try to refrain from commenting on topics outside your expertise.