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I believe that the concentration of the solution they were suspended in was 80 uM, but the concentration in their blood isn't mentioned (and probably wasn't mea

by Midfit 4y ago

I believe that the concentration of the solution they were suspended in was 80 uM, but the concentration in their blood isn't mentioned (and probably wasn't measured) in their work. I'm not a zebrafish expert so I have no idea how much PFAS would a zebrafish absorb from its surrounding solution into its bloodstream.

Mistletoe 4y ago
It says the embryos and cells were put in 80 uM. >The embryos and cells were exposed to 80 micromolar solutions of each chemical:
- Midfit 4y ago
  Yes, but your original comment talks about a concentration of 6 ng/mL in human blood, while the researchers only point out the concentration of the surrounding medium. Perhaps I'm missing something, but you can't compare these two values since you didn't measure the actual concentration of PFAS in the blood stream of the larvae. For all we know it could be lower than 6 ng/mL, right?
  - Mistletoe 4y ago
    They are also doing studies on human neutrophil cells etc. at 80 uM. The fluid above the cells at 80 uM is a model for cells in blood. You can see in Figure 5 here that the effects are really only seen at the highest non physiological doses. Even their lowest dose of GenX is 2.6 uM which is 2600 nM. I don’t have data for blood levels of GenX but if it is like PFOA that would be 260x higher than any concentration we would expect a neutrophil to ever see. And there is no effect on respiratory burst. https://www.tandfonline.com/doi/epdf/10.1080/1547691X.2023.2176953?needAccess=true&role=button https://www.tandfonline.com/doi/epdf/10.1080/1547691X.2023.2...