3 ms·
Computational biologist with >5 years experience in analyzing RNAseq data like the one in the paper: I find 80% of genes being differentially expressed very har
by aquafox 4y ago
Computational biologist with >5 years experience in analyzing RNAseq data like the one in the paper: I find 80% of genes being differentially expressed very hard to believe. You usually see such strong effects only when there are other sources of variation not being controlled for (e.g. batch effects) or when inducing an unphysiological state (e.g. treating with a cytotoxic drug).
- manmal 4y agoHave you considered that 16h for a mouse might, from a metabolic POV, be comparable to 4+ days in humans? That’d be quite the intervention.
- 331c8c71 4y agoI think they are overselling... Not clear to me how they treated the multiple testing across 22 tissues - gene expression measurements across different tissues in a single mouse are clearly not independent from each other. > A total of 15,430 genes were DE in at least one tissue, but only 816 and 1,335 genes were differentially up- or downregulated, respectively, by TRF in ≥5 tissues