4 ms·
As an experimental neuroscientist that has recorded fairly extensively from brain organoids, I would advise that these are HIGHLY underdeveloped cultures. When
by tbenst 4y ago
As an experimental neuroscientist that has recorded fairly extensively from brain organoids, I would advise that these are HIGHLY underdeveloped cultures. When patch clamping, I had to depolarize neurons in a human brain organoid to -25mV to trigger action potentials (normally -60mV is sufficient; healthy neurons resting membrane potential is -70mV) Despite imaging organoids for hours with light sheet—a functional imaging technique that allows for observation of nearly all neurons in the organoid simultaneously, I did not observe any spontaneous action potentials, only diffusive waves of calcium activity.
Of course, not all organoids are created equal, and the protocols around extracellular matrices are improving constantly, but for folks interested in systems neuroscience the organoid field is still too underdeveloped to ask interesting questions around functional activity.
- iamwizard 4y agoAs someone who recently left the field of experimental neuroscience, anecdotes like this hit at why media coverage of neuroscience is so sensationalized and reductive: it takes a lot of nuance and background to convey the impact of any given finding. In a lot of cases being even slightly reductive of EXACTLY what was reported immediately destroys the meaning and context of a finding. It's a field that actively resists compression of information and is so heterogeneously interdisciplinary that having the correct context to interpret a finding even within the field is often difficult.
- afpx 4y agoWhy did you leave the field? The compensation?
- iamwizard 4y agoThat was certainly part of it. The structure of neuro academia meant that I would be stuck in a postdoc position for 3-10 years paying, likely, around the NIH minimum of around $55k. Which goes along with more responsibility and drastically less job security than a graduate student without any better chances of actually getting a faculty position. Combine that with the fact that I didn't have the drive or disposition that the field selects for and I was out of there immediately after I got my degree for a more lucrative, more interesting (to me) job with a better work-life balance.