4 ms·
You'd need: fresh fertilized chicken eggs lab gloves an egg incubator a dissecting microscope tissue culture dishes phosphate-buffered saline a pipette p
by twic 4y ago
You'd need:
fresh fertilized chicken eggs
lab gloves
an egg incubator
a dissecting microscope
tissue culture dishes
phosphate-buffered saline
a pipette pump
10 ml pipettes
two pairs of dissecting tweezers
70% ethanol solution
kimwipes
lab gloves
a steady hand
tissue culture medium (DMEM is fine)
fetal calf serum
penicillin-streptomycin mix
a micropipette
micropipette tips
a tissue culture incubator
a tank of carbon dioxide
ideally, a laminar flow cabinet, but reasonably careful technique and the pen/strep would probably see you through
So, when? Depends if you have Prime really.
The thing is, growing sheets of cells is not a big step towards doing anything interesting. You need to do chemical and genetic manipulation of the cells, and that's the hard part.