4 ms·
Unfortunately, however, FASTQ files have more characters than ACGT and (N). They include a second line that encodes sequence quality. In practice, that line is
by fastaguy88 4y ago
Unfortunately, however, FASTQ files have more characters than ACGT and (N). They include a second line that encodes sequence quality. In practice, that line is even more compressible, but it complicates things.
(And of course the label on each entry is just standard 7-bit ascii), and also very compressible, but it contains higher complexity text.
- jefftk 4y ago> In practice, that line is even more compressible, but it complicates things. It depends: for some platforms (ex: NextSeq) the quality score in binned and highly compressible, but for others (ex: Nanopore) it's much higher entropy than the sequence.