3 ms·

This isn’t a great way to test this. It’s a good way to begin exploring it. >Enterocytic liquid-liquid interfaces were established on permeable supports http

This isn’t a great way to test this. It’s a good way to begin exploring it. >Enterocytic liquid-liquid interfaces were established on permeable supports https://bioduro-sundia.com/wp-content/uploads/2017/09/caco-2-assay.jpg https://bioduro-sundia.com/wp-content/uploads/2017/09/caco-2... This is like a fake very fragile layer of cells cultured on a membrane. It’s good for preliminary data but it needs follow up data in vivo. Caveats off the top of my head- 1. Your real gut is massively more robust than Caco-2 cells grown as a monolayer on a support. You’ve got blood supply, lympathic system, villi and connective tissue, layer upon layer of cells covered in mucus- just a completely different ballgame. 2. Rinse aid would first go through your stomach, exposed to acid, be absorbed maybe, have pancreatic secretions added to it, have food with it and all the lipids, very long list. 3. What’s the concentration of rinse aid you actually absorb from your dishes? 1:20,000 doesn’t seem like enough dilution to me but maybe they address that. Nevertheless I won’t use rinse aid again. :) It’s not necessary for my dishes and it isn’t even worth the cost.

twic 4y ago
The dilutions seem unrealistic, or at least physiologically irrelevant. This is how they get there: > A professional dishwasher completes 1 or 2 wash and rinse cycles using 3.5 L of water per cycle. The detergent and rinse aid are automatically dispensed into the water at a concentration of 1.5 to 4 mL/L and 0.1 to 0.5 mL/L, respectively. At these concentrations, the residual dilution factor after rinse ranges from 1:250 to 1:667 for detergents and 1:2,000 to 1:10,000 for rinse aids. So 1:10,000 is a plausible dilution of rinse aid in the rinse water. Unless people are drinking the rinse water (please don't), it's not a dilution that will ever be seen by the body. Rather, what the body will see is however much residue is left on a mug diluted in whatever the volume of the mug is. They do sort of address this: > To study the chemicals in the residue remaining on the surface of dishware after washing, 10 porcelain cups (190 mL size) were subjected to 1 full washing and drying cycle in professional (Winterhalter, GS501, Germany) and household (AEG, GS60AV, Poland) dishwashers and then 1 mL of culture medium was added into them. After shaking and waiting for 5 minutes, the culture medium was collected from the cups, filter-sterilized, and added to the monolayer and differentiated cell cultures directly or in 1:2, 1:5, and 1:10 dilutions. Except that they wash all the residues off in just 1 ml of medium. They then dilute that 1 ml onto cells: > The LDH cytotoxicity assay on monolayer Caco-2 cells showed that the toxicity exerted by the dried rinse aid residue on the cups washed in a professional dishwasher still takes place even at a 1:10 dilution (see Fig E9, A). TEER and PF were also measured in differentiated Caco-2 cells, and parallel results were observed when exposed to rinse aid. High concentrations (1:2 and 1:5 dilutions) of the residue severely impaired the epithelial barrier function as measured by the decreased TEER values. In the 1:10 dilution, nearly the same TEER values were observed compared with controls. So this isn't great, but it also doesn't seem like a big deal. You could rinse a cup in 10 ml of water, gargle with it, and be fine.

twic 4y ago

The dilutions seem unrealistic, or at least physiologically irrelevant. This is how they get there: > A professional dishwasher completes 1 or 2 wash and rinse cycles using 3.5 L of water per cycle. The detergent and rinse aid are automatically dispensed into the water at a concentration of 1.5 to 4 mL/L and 0.1 to 0.5 mL/L, respectively. At these concentrations, the residual dilution factor after rinse ranges from 1:250 to 1:667 for detergents and 1:2,000 to 1:10,000 for rinse aids. So 1:10,000 is a plausible dilution of rinse aid in the rinse water. Unless people are drinking the rinse water (please don't), it's not a dilution that will ever be seen by the body. Rather, what the body will see is however much residue is left on a mug diluted in whatever the volume of the mug is. They do sort of address this: > To study the chemicals in the residue remaining on the surface of dishware after washing, 10 porcelain cups (190 mL size) were subjected to 1 full washing and drying cycle in professional (Winterhalter, GS501, Germany) and household (AEG, GS60AV, Poland) dishwashers and then 1 mL of culture medium was added into them. After shaking and waiting for 5 minutes, the culture medium was collected from the cups, filter-sterilized, and added to the monolayer and differentiated cell cultures directly or in 1:2, 1:5, and 1:10 dilutions. Except that they wash all the residues off in just 1 ml of medium. They then dilute that 1 ml onto cells: > The LDH cytotoxicity assay on monolayer Caco-2 cells showed that the toxicity exerted by the dried rinse aid residue on the cups washed in a professional dishwasher still takes place even at a 1:10 dilution (see Fig E9, A). TEER and PF were also measured in differentiated Caco-2 cells, and parallel results were observed when exposed to rinse aid. High concentrations (1:2 and 1:5 dilutions) of the residue severely impaired the epithelial barrier function as measured by the decreased TEER values. In the 1:10 dilution, nearly the same TEER values were observed compared with controls. So this isn't great, but it also doesn't seem like a big deal. You could rinse a cup in 10 ml of water, gargle with it, and be fine.