Their description of the assembly strategy as "Golden Gate" seems like incorrect terminology. The WIV has at least published papers using BsaI and BsmBI, though

Their description of the assembly strategy as "Golden Gate" seems like incorrect terminology. The WIV has at least published papers using BsaI and BsmBI, though. https://twitter.com/jbkinney/status/1583267221047869441 https://twitter.com/jbkinney/status/1583267221047869441 https://twitter.com/jbkinney/status/1583248052969562112 https://twitter.com/jbkinney/status/1583248052969562112 https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006698 https://journals.plos.org/plospathogens/article?id=10.1371/j... EDIT: Kinney also asserts here that they left the sites in a final assembled genome (i.e., the genome of a replication-competent virus). I'm still trying to figure out if that's true, though.