2 ms·
The first step of a western is SDS-PAGE.
by throwawaymaths 4y ago
The first step of a western is SDS-PAGE.
- DrAwdeOccarim 4y agoTo follow on, typically proteins produced this way have a tag, or handle, to specifically pull it out from all the other proteins. A typica tag is 6 x Histidine amino acids at the beginning of the protein. These histidines bind to nickel, which is immobilized on a resin. You can use antibodies against the 6xHis to Western for your protein of desire without needing a protein specific antibody.