SDS-PAGE is the normal way, at least for an initial test expression. For a Western blot or ELISA you need antibodies specific to your protein, which you probabl

SDS-PAGE is the normal way, at least for an initial test expression. For a Western blot or ELISA you need antibodies specific to your protein, which you probably don’t have. An easier way to identify, for example, would be to cut the SDS-PAGE band and perform mass spectrometry on it to try to elucidate its protein sequence.