No, and yes. The difference is obvious even those who don't distinguish pitches much (can you hear the difference in the video linked in the article - https://

No, and yes. The difference is obvious even those who don't distinguish pitches much (can you hear the difference in the video linked in the article - https://www.youtube.com/watch?v=7GhAuZH6phs&t=21s https://www.youtube.com/watch?v=7GhAuZH6phs&t=21s ) because the 'wrong' one played in a scale or a chord sounds really, really horrible; that's why we have all the work done on 'tempered' tuning to reduce the gap so that everything is just slightly off.