3 ms·
Not for home gamers, no. ONT is fairly low throughput and the prep is surprisingly difficult to do well without experience. You would need to run tens of flow c
by aroch 4y ago
Not for home gamers, no. ONT is fairly low throughput and the prep is surprisingly difficult to do well without experience. You would need to run tens of flow cells to get enough sequencing data.
- mbreese 4y agoNot to mention there is a good chunk of required "extra" equipment that is expected (centrifuges, etc) that bring the initial cost to well over $1000. You could buy most thing used, but some of the reagents could be difficult to acquire outside of a traditional lab.
- ejstronge 4y agoThis is not strictly true - there is a transposase based prep that does not require special equipment. Still, ONT users would need to understand how to convert raw reads into useable basecalls at loci of clinical interest
- mbreese 4y agoDoes it still require a pipettor, or could it work with just a transfer pipet? Their field kit should probably be as minimal as it could be. Last I looked into it, it was a minimal set of extra equipment -- votexer, centrifuge, etc... nothing unusual for a bio lab. And even most of that could be handled with a good snap of the wrist, if you weren't trying to be super precise.
- ejstronge 4y agoIf one is patient, the only tool needed in the wet lab is a transfer pipettor and a scale - I think this could be done without a micropipettor