Not trans myself, but I can tell you from my personal experience that very few people are actually uncomfortable or alienated by topics like this – that's usual

Not trans myself, but I can tell you from my personal experience that very few people are actually uncomfortable or alienated by topics like this – that's usually more of a bit of fiction belonging to the "you can't even say WOMAN any more" genre. In contrast, trans people are usually extremely aware of their biology and related medical requirements - to a greater extent than many others. Like any other topic that might be sensitive, the best approach is to be respectful, accurate, and specific. For example, don't do the thing up-thread of banging on about how "gender isn't relevant to this conversation" without really having that insight.