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> I say this because they used a new sequencing technology that appears to have homopolymer bias (https://twitter.com/lpachter/status/1533875723995185153 https:
by cauthon 4y ago
> I say this because they used a new sequencing technology that appears to have homopolymer bias (https://twitter.com/lpachter/status/1533875723995185153 https://twitter.com/lpachter/status/1533875723995185153), which will bias the gene quantification.
I believe they used Illumina for the results presented in the main text and then re-sequenced with Ultima and replicated a subset of the analyses (fig s13). The Ultima proof-of-concept didn't appear to be relevant to the main study/conclusions