Depends if the bot is using full browser emulation or not. If they're using Puppeteer or something to drive a headless browser, it seems likely the ad would be

Depends if the bot is using full browser emulation or not. If they're using Puppeteer or something to drive a headless browser, it seems likely the ad would be recorded as "shown". If they're just making requests to specific endpoints sufficient to avoid Twitter's bot countermeasures, then it's likely not registering as an ad impression.