4 ms·
Just be careful with the ethidium bromide.
by bedris 15y ago
Just be careful with the ethidium bromide.
- nazar 15y agoThat what lab assistant usually says. At uni we used some blue florescent dye that apparently got ionically bonded to P group of DNA backbone. It was completely safe(not carcinogenic). I can't remember the name of the dye, but results with EtBr were much clearer.
- tomkinstinch 15y agoGelRed is another safe one. It is designed so that it will not cross cell membranes. I don't think it intercalates either.
- RoyalSWiSH 15y agoWhat about Methylen blue? Do you guys have an idea if this will work? Read it somewhere. But i don't think it is florescent.
- nazar 15y agoIf my memory doesn't lie to me, I think its a load dye, which only dyes the DNA sample to visually assist during the loading of the samples into the "wells" of the gel. Otherwise you can't be sure whether you put it inside the well or somewhere into the surrounding buffer.
- mbreese 15y agoDepending on what hazmat disposal they have, they'd be better off using something like SYBR Safe, especially with beginners. It's almost as good as EtBr for staining, but not toxic. (Downside: it's much more expensive, EtBr is cheap as hell)
- tomkinstinch 15y agoEtBr is cheap as hell, but the hazmat disposal needed to get rid of EtBr gels is very expensive.