3 ms·
Yeah, I did a janky cardboard box and saran wrap glovebox when I tried to do my own innoculation... was a PITA to use, and contamination from that batch was wor
by cwkoss 5y ago
Yeah, I did a janky cardboard box and saran wrap glovebox when I tried to do my own innoculation... was a PITA to use, and contamination from that batch was worse than when I just went into a room with still air and crossed my fingers.
(didnt have HEPA filtered air blowing, that sounds like a good idea)
- wy35 5y agoA big plastic tub with two arm holes cut out would be a much better solution. And I hope by “glovebox” you mean a still-air box, because gloves attached to the box actually increase the contamination rate due to the box sucking in air when you move your hands.
- cwkoss 5y agoyep, still air box is the correct term I think. It was a cardboard box with two armholes cut, and saran wrap stretched over the top. Put all my equipment inside, stuck my arms in to more or less plug the holes, then sprayed a bunch of rubbing alcohol all over the inside, then got to work. The ergonomics were horrible, my back got tired, and I'm pretty sure my arms didn't plug the holes very well and I assume they effectively were pumping air through the system by the end of it as I got sloppier. Definitely will do something better on any future attempts, haha
- wy35 5y agoThe easiest technique is getting a big plastic tub (>90 qts) from Walmart or Target, heating up a coffee can on the stove, and burning the arm holes in the side.