(3 minutes * (440 / 432 - 1)) * 24 FPS = 80 frames You're not wrong that you wouldn't be able to tell the difference as long as you don't have perfect pitch (i

(3 minutes * (440 / 432 - 1)) * 24 FPS = 80 frames You're not wrong that you wouldn't be able to tell the difference as long as you don't have perfect pitch (it's a fraction of a semitone), but you might want to check your math.