3 ms·
How do you know the quality of the resulting sequence?
by pas 5y ago
How do you know the quality of the resulting sequence?
- ampdepolymerase 5y agoYou don't, not without either resequencing it with another sequencing system or benchmarking the sequencer with a known sequence.
- deleted 5y ago[deleted]
- joshuamcginnis 5y agoThere are quality checks throughout the entire process, starting from the raw read quality scores returned directly from the sequencer all the way to fully assembled genome completeness. In our paper, one of the tools we used for this is called BUSCO[0] which scored our assembly at 97.9%, a relatively high score for de novo assemblies. [0] https://pubmed.ncbi.nlm.nih.gov/31020564/ https://pubmed.ncbi.nlm.nih.gov/31020564/