There's a lot of ways you can get fancier and more precise quantitative results, sure. The basics is the cycle threshold. The next step further is just to try

There's a lot of ways you can get fancier and more precise quantitative results, sure. The basics is the cycle threshold. The next step further is just to try and come up with a fractional cycle number through interpolation-- e.g. if cycle 20 was not-quite-positive-but-very-close and cycle 21 was strongly positive, that implies a value close to 20, versus if cycle 20 was very far from positive and 21 was just barely over the threshold, that implies the real value is just under 21. And there's further, but diminishing gains from fitting the fluorescence curves.