Don't forget having multiple images in one file - 200.000x200.000 pixel main tiled, pyramidal jpeg or jp2 encoded image, thumbnail, label, overview and barcode.

Don't forget having multiple images in one file - 200.000x200.000 pixel main tiled, pyramidal jpeg or jp2 encoded image, thumbnail, label, overview and barcode. Next to the standardized metadata like resolution and vendor-specific xml with descriptions of channels, wavelengths, antibodies and complete provenance. With libtiff and libtiff-tools to help you make sense of it.