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I am surprised by your comment. We have much better techniques for understanding off-target issues with DSB-inducing gene editors. I'm certain the tox package $
by DrAwdeOccarim 5y ago
I am surprised by your comment. We have much better techniques for understanding off-target issues with DSB-inducing gene editors. I'm certain the tox package $NTLA put together to support NTLA-2001 looked into this non-clinically very, very closely.
From my vantage point, $BEAM does not have the analytical tools to actually know where edits are occurring in an entire genome-context. Maybe RCA-type ideas, or super deep NGS, but I haven't seen robust and convincing off-target analysis yet.
- xiphias2 5y agoI don't have enough biological knowledge to understand the potential off-target effects of current base editing technology at $BEAM, and I accept your opinion that it's not yet ready for production, but in general what I love about base editing is that there are billions of people living as control / experiment groups, so as long as the off-target effects can be understood and minimized (hopefully in a few years), I don't have to worry too much about the main effect too much.